Integrated Transcriptome and Metabolome Analysis Reveal That Exogenous Gibberellin Application Regulates Lignin Synthesis in Ramie

Gibberellin regulates plant growth, development, and metabolic processes. However, the underlying mechanism of substantial effect gibberellin on stem height secondary metabolites in forage ramie is unclear. Therefore, this study combined transcriptomic metabolomics analyses to identify mechanisms regulating growth metabolite contents following exogenous application. Exogenous application significantly reduced lignin content leaves but not stems. At same time, increased total flavonoid chlorogenic acid both stems leaves. In addition, 293 differentially expressed genes (DEGs) 68 (DEMs) were identified stems, 128 DEGs 41 DEMs identified. The PER42, FLS, CYP75A, PNC1 up-regulated leaves, affecting phenylpropane metabolism. joint analysis revealed that changes improved substrate efficiency phenol propane pathway inhibited synthesis plants, thus shifting synthesis. conclusion, treatment effectively reduces while increasing contents. These findings provide empirical practical guidance for breeding quality improvement cultivation control ramie.